Journal: PLOS ONE

Article Title: Recovery after human bone marrow mesenchymal stem cells (hBM-MSCs)-derived extracellular vesicles (EVs) treatment in post-MCAO rats requires repeated handling

doi: 10.1371/journal.pone.0312298

Figure Lengend Snippet: (a–c) Characterization and quantification of human-bone marrow mesenchymal stem cell (hBM-MSC)-derived extracellular vesicles (EVs). (a) Nanosight Tracking analyses (NTA) showing the size distribution pattern of EVs. (b) Western blots of hBM-MSCs marker (GM130) and EVs markers (ALIX, CD9, CD81). “Wash” lane represents the negative control. Each image comes from different blots. (c) Representative image of EVs using transmission electron microscopy (TEM). Scale bar 100 nm. (d–f) In vivo tracking of ExoGlow-labeled EVs demonstrated that EVs reach the brain and accumulate near the stroke injury 6 hours after administration at 2 dps. (d) Timeline of intranasal single dose treatment of ExoGlow-labeled EVs (~2.4 x 10 9 EVs in 200 μl), starting 2 dps and collecting the tissue 6 hours after. (e) Representative coronal section after a single intranasal administration of ExoGlow-labeled EVs (~2.4 x 10 9 EVs in 200 μl) at 2 dps. Hoechst indicates cell nuclei. Scale bars 4 mm and 100 μm. (f) Quantification of the average size of ExoGlow+ particles in the ipsilateral and contralateral hemispheres. Data was analyzed using a paired t-test (*p<0.05). Bars show mean +/− SD. Each symbol represents the average size of ExoGlow+ particles from all ROIs in each hemisphere (5 ROIs/hemisphere in each rat) of a single rat ( n = 4 ).

Article Snippet: Human bone marrow mesenchymal stem cells (hBM-MSCs) were purchased from Celprogren (frozen vial with ~1.2 x 10 6 cells; #36094–22) and plated on human mesenchymal bone marrow stem cell culture extracellular expansion matrix pre-coated T75 Flasks (Celprogen, #E36094-21-T75) following Celprogren recommendations.

Techniques: Derivative Assay, Western Blot, Marker, Negative Control, Transmission Assay, Electron Microscopy, In Vivo, Labeling

Journal: Advances in Wound Care

Article Title: A Small Chimeric Fibronectin Fragment Accelerates Dermal Wound Repair in Diabetic Mice

doi: 10.1089/wound.2015.0666

Figure Lengend Snippet: FNIII1HRGD supports MSC adhesion and proliferation. Tissue culture plates were precoated with increasing concentrations of FNIII1HRGD (50–400 nM). MSCs were seeded (1 × 104 cells/cm2) onto protein-coated plates. (A) Phase contrast microscopy images were taken at 72 h postseeding. Images represent one of three experiments performed. Scale bar, 50 μm. (B) Cell number was determined at 1, 4, and 7 days postseeding using crystal violet, as described in the Materials and Methods section. Data are presented as mean absorbance ± SEM of three experiments performed in triplicate. *Significantly different from FNIII1HRGD at corresponding time point, p < 0.05 (ANOVA). MSC, mesenchymal stem cell.

Article Snippet: 21 Human mesenchymal stem cells (MSCs) and MSC Growth Medium were from Lonza.

Techniques: Microscopy